anti-ccl22 neutralizing antibodies Search Results


sup-t1  (ATCC)
97
ATCC sup-t1
Sup T1, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti human ccl22 mdc neutralizing ab  (R&D Systems)
92
R&D Systems anti human ccl22 mdc neutralizing ab
Anti Human Ccl22 Mdc Neutralizing Ab, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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neutralizing anti-ccl22  (R&D Systems)
90
R&D Systems neutralizing anti-ccl22
Neutralizing Anti Ccl22, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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goat anti mouse ccl22 igg  (R&D Systems)
93
R&D Systems goat anti mouse ccl22 igg
(A) mRNA transcripts for the three DC chemoattractant chemokines, <t>CCL22,</t> CCL8 and CCL20, were assayed by real time RT-PCR in the colon of B6 and GM-CSF-/- mice left uninfected or infected with C. rodentium (n = 4-5 mice per group at each time point). (B) CCL22 protein was assayed in colon extracts from B6 and GM-CSF-/- mice. Data are mean ± SEM (n = 5 mice per group at each time point). For (A, B) * p < 0.05 relative to B6 mice at the indicated time points. (C) Colon sections from B6 mice left uninfected and B6 and GM-CSF-/- mice infected for 3 weeks with C. rodentium were stained for CCL22 (red). Original magnification, x 200. (D) Colon sections from B6 mice infected for 3 weeks were stained for CCL22 (red) and β-catenin (green) as an epithelial cell marker. Nuclei were stained with Hoechst 33258 (blue). Specificity controls using control antibody or anti-CCL22 absorbed with CCL22 peptide showed no immunostaining (see Fig. S3). (E) Colon sections from B6 mice infected for 3 weeks were stained for CCL22 (red) and CD11c (green). Original magnification, x 400. (F) WT B6 mice were infected with C. rodentium for 2 weeks. On days 9, 11 and 13 after infection mice were injected i.p. with 20 μg IgG goat anti-CCL22 or control goat IgG.. Colon sections from anti-CCL22 and control goat IgG treated mice were stained for CD11c (red) and F-actin (green). Original magnification, x 200. (G) Cells isolated from colon lamina propria, spleen, and MLN of anti-CCL22 and control goat IgG treated mice were characterized by flow cytometry. Similar results were obtained in 2 independent experiments.
Goat Anti Mouse Ccl22 Igg, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat anti mouse ccl22 igg/product/R&D Systems
Average 93 stars, based on 1 article reviews
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polyclonal goat anti-mouse mdc antibody  (R&D Systems)
90
R&D Systems polyclonal goat anti-mouse mdc antibody
(A) mRNA transcripts for the three DC chemoattractant chemokines, <t>CCL22,</t> CCL8 and CCL20, were assayed by real time RT-PCR in the colon of B6 and GM-CSF-/- mice left uninfected or infected with C. rodentium (n = 4-5 mice per group at each time point). (B) CCL22 protein was assayed in colon extracts from B6 and GM-CSF-/- mice. Data are mean ± SEM (n = 5 mice per group at each time point). For (A, B) * p < 0.05 relative to B6 mice at the indicated time points. (C) Colon sections from B6 mice left uninfected and B6 and GM-CSF-/- mice infected for 3 weeks with C. rodentium were stained for CCL22 (red). Original magnification, x 200. (D) Colon sections from B6 mice infected for 3 weeks were stained for CCL22 (red) and β-catenin (green) as an epithelial cell marker. Nuclei were stained with Hoechst 33258 (blue). Specificity controls using control antibody or anti-CCL22 absorbed with CCL22 peptide showed no immunostaining (see Fig. S3). (E) Colon sections from B6 mice infected for 3 weeks were stained for CCL22 (red) and CD11c (green). Original magnification, x 400. (F) WT B6 mice were infected with C. rodentium for 2 weeks. On days 9, 11 and 13 after infection mice were injected i.p. with 20 μg IgG goat anti-CCL22 or control goat IgG.. Colon sections from anti-CCL22 and control goat IgG treated mice were stained for CD11c (red) and F-actin (green). Original magnification, x 200. (G) Cells isolated from colon lamina propria, spleen, and MLN of anti-CCL22 and control goat IgG treated mice were characterized by flow cytometry. Similar results were obtained in 2 independent experiments.
Polyclonal Goat Anti Mouse Mdc Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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human ccl22/mdc antibody  (Bio-Techne corporation)
94
Bio-Techne corporation human ccl22/mdc antibody
(A) mRNA transcripts for the three DC chemoattractant chemokines, <t>CCL22,</t> CCL8 and CCL20, were assayed by real time RT-PCR in the colon of B6 and GM-CSF-/- mice left uninfected or infected with C. rodentium (n = 4-5 mice per group at each time point). (B) CCL22 protein was assayed in colon extracts from B6 and GM-CSF-/- mice. Data are mean ± SEM (n = 5 mice per group at each time point). For (A, B) * p < 0.05 relative to B6 mice at the indicated time points. (C) Colon sections from B6 mice left uninfected and B6 and GM-CSF-/- mice infected for 3 weeks with C. rodentium were stained for CCL22 (red). Original magnification, x 200. (D) Colon sections from B6 mice infected for 3 weeks were stained for CCL22 (red) and β-catenin (green) as an epithelial cell marker. Nuclei were stained with Hoechst 33258 (blue). Specificity controls using control antibody or anti-CCL22 absorbed with CCL22 peptide showed no immunostaining (see Fig. S3). (E) Colon sections from B6 mice infected for 3 weeks were stained for CCL22 (red) and CD11c (green). Original magnification, x 400. (F) WT B6 mice were infected with C. rodentium for 2 weeks. On days 9, 11 and 13 after infection mice were injected i.p. with 20 μg IgG goat anti-CCL22 or control goat IgG.. Colon sections from anti-CCL22 and control goat IgG treated mice were stained for CD11c (red) and F-actin (green). Original magnification, x 200. (G) Cells isolated from colon lamina propria, spleen, and MLN of anti-CCL22 and control goat IgG treated mice were characterized by flow cytometry. Similar results were obtained in 2 independent experiments.
Human Ccl22/Mdc Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human ccl22/mdc antibody/product/Bio-Techne corporation
Average 94 stars, based on 1 article reviews
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Image Search Results


(A) mRNA transcripts for the three DC chemoattractant chemokines, CCL22, CCL8 and CCL20, were assayed by real time RT-PCR in the colon of B6 and GM-CSF-/- mice left uninfected or infected with C. rodentium (n = 4-5 mice per group at each time point). (B) CCL22 protein was assayed in colon extracts from B6 and GM-CSF-/- mice. Data are mean ± SEM (n = 5 mice per group at each time point). For (A, B) * p < 0.05 relative to B6 mice at the indicated time points. (C) Colon sections from B6 mice left uninfected and B6 and GM-CSF-/- mice infected for 3 weeks with C. rodentium were stained for CCL22 (red). Original magnification, x 200. (D) Colon sections from B6 mice infected for 3 weeks were stained for CCL22 (red) and β-catenin (green) as an epithelial cell marker. Nuclei were stained with Hoechst 33258 (blue). Specificity controls using control antibody or anti-CCL22 absorbed with CCL22 peptide showed no immunostaining (see Fig. S3). (E) Colon sections from B6 mice infected for 3 weeks were stained for CCL22 (red) and CD11c (green). Original magnification, x 400. (F) WT B6 mice were infected with C. rodentium for 2 weeks. On days 9, 11 and 13 after infection mice were injected i.p. with 20 μg IgG goat anti-CCL22 or control goat IgG.. Colon sections from anti-CCL22 and control goat IgG treated mice were stained for CD11c (red) and F-actin (green). Original magnification, x 200. (G) Cells isolated from colon lamina propria, spleen, and MLN of anti-CCL22 and control goat IgG treated mice were characterized by flow cytometry. Similar results were obtained in 2 independent experiments.

Journal:

Article Title: GM-CSF promoted DC recruitment and survival governs the intestinal mucosal response to enteric attaching-and-effacing bacterial pathogens

doi: 10.1016/j.chom.2010.01.006

Figure Lengend Snippet: (A) mRNA transcripts for the three DC chemoattractant chemokines, CCL22, CCL8 and CCL20, were assayed by real time RT-PCR in the colon of B6 and GM-CSF-/- mice left uninfected or infected with C. rodentium (n = 4-5 mice per group at each time point). (B) CCL22 protein was assayed in colon extracts from B6 and GM-CSF-/- mice. Data are mean ± SEM (n = 5 mice per group at each time point). For (A, B) * p < 0.05 relative to B6 mice at the indicated time points. (C) Colon sections from B6 mice left uninfected and B6 and GM-CSF-/- mice infected for 3 weeks with C. rodentium were stained for CCL22 (red). Original magnification, x 200. (D) Colon sections from B6 mice infected for 3 weeks were stained for CCL22 (red) and β-catenin (green) as an epithelial cell marker. Nuclei were stained with Hoechst 33258 (blue). Specificity controls using control antibody or anti-CCL22 absorbed with CCL22 peptide showed no immunostaining (see Fig. S3). (E) Colon sections from B6 mice infected for 3 weeks were stained for CCL22 (red) and CD11c (green). Original magnification, x 400. (F) WT B6 mice were infected with C. rodentium for 2 weeks. On days 9, 11 and 13 after infection mice were injected i.p. with 20 μg IgG goat anti-CCL22 or control goat IgG.. Colon sections from anti-CCL22 and control goat IgG treated mice were stained for CD11c (red) and F-actin (green). Original magnification, x 200. (G) Cells isolated from colon lamina propria, spleen, and MLN of anti-CCL22 and control goat IgG treated mice were characterized by flow cytometry. Similar results were obtained in 2 independent experiments.

Article Snippet: The following polyclonal antibodies were used: goat anti-mouse and rat CCL22 (IgG) from Santa Cruz for immunostaining experiments, goat anti-mouse CCL22 (IgG) from R&D systems for in vivo neutralization experiments, rabbit anti-FITC (Alexa 488-conjugated, IgG) from Molecular Probes.

Techniques: Quantitative RT-PCR, Infection, Staining, Marker, Control, Immunostaining, Injection, Isolation, Flow Cytometry